RESUMEN
Objective: In vitro maturation technique [IVM] is shown to have an effect on full maturation of immature oocytes and the subsequent embryo development. Embryonic genome activation [EGA] is considered as a crucial and the first process after fertilization. EGA failure leads to embryo arrest and possible implantation failure. This study aimed to determine the role of IVM in EGA-related genes expression in human embryo originated from immature oocytes and recovered from women receiving gonadotrophin treatment for assisted reproduction
Materials and Methods: In this experimental study, germinal vesicle [GV] oocytes were cultured in vitro. After intracytoplasmic sperm injection of the oocytes, fertilization, cleavage and embryo quality score were assessed in vitro and in vivo. After 3-4 days, a single blastomere was biopsied from the embryos and then frozen. Afterwards, the expression of EGA-related genes in embryos was assayed using quantitative reverse transcriptase-polymerase chain reaction [PCR]
Results: The in vitro study showed reduced quality of embryos. No significant difference was found between embryo quality scores for the two groups [P=0.754]. The in vitro group exhibited a relatively reduced expression of the EGA- related genes, when compared to the in vivo group [all of them showed P=0.0001]
Conclusion: Although displaying the normal morphology, the IVM process appeared to have a negative influence on developmental gene expression levels of human preimplanted embryos. Based on our results, the embryo normal morphology cannot be considered as an ideal scale for the successful growth of embryo at implantation and downstream processes
RESUMEN
The aim of this study was to evaluate and compare the population of Natural killer T lymphocyte [NKT] in the uterus and spleen of the hyperstimulated and control mice at the seventh day of pregnancy. The superovulated and control mice were put individually in a cage with a male mouse. In the next morning they were considered for observing the vaginal plag. The day was assumed as the first day of pregnancy. On the 7th day of pregnancy, the samples were collected from uterus of implantation site, interval site and spleen tissues and 5 micrometer cryosections were prepared. The immunohistochemical reaction was used for CD 161 and CD3 markers and the distribution of NKT cells population were compared with nucleated cells in hyperstimulated and control groups. There were not significant differences between the NKT cell population of the spleen, decidual and myometrial tissue of interval site in the control and hyperstimulated groups. But this population was increased in the hyperstimulated group [2.26 +/- 1.43] compared with control [0.79 +/- 0.17; P